Cat. No.: SCELL-12175

U2OS Cells stably expressing M5 Muscarinic Receptor and Diacylglycerol Biosensor

Description DAG NOMAD Muscarinic M5 Receptor (M5R) cell line stably co-expresses label-free GPCR and diacylglycerol (DAG) NOMAD biosensor, which monitors G protein regulation through DAG release.
Tested applications Suitable for: ICC, WB
Size 2 vials, 3*106 cells/vial
Format Frozen
Shipping Info Dry Ice
Quality Control All cells test negative for bacteria, yeast, and fungi.
Storage Conditions Liquid Nitrogen
Categories Engineered Cell Lines, Multiplexing GPCR Screening Cell Lines - NOMAD
Tags Diacylglycerol (DAG) Biosensor, M5 Muscarinic Receptor Stable Cell Line, M5R-DAG Stable Cell Line, NOMAD Cell Line
Culture Condition 37℃ with 5% CO2
Applications For Research Use Only
Product Type Stable Cell Line
Mycoplasma free Yes
Subculture Guidelines The recommended seeding density is 2*104 cells/cm2.
If necessary, a partial replacement of the medium 24 hours before subculture may help promote growth.
When the cells achieve 80-90% confluence, they should be passaged.
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